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SKU: PKSH030448_100μg-ES
Recombinant Human CD4/LEU3 Protein (His Tag), 100 μg
T-cell surface glycoprotein CD4, is a single-pass type I membrane protein. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. The CD4 surface determinant, previously associated as a phenotypic marker for helper/inducer subsets of T lymphocytes, has now been critically identified as the binding/entry protein for human immunodeficiency viruses (HIV). The human CD4 molecule is readily detectable on monocytes, T lymphocytes, and brain tissues. CD4 is a co-receptor that assists the T cell receptor (TCR) to activate its T cell following an interaction with an antigen presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR. CD4 interacts directly with MHC class II molecules on the surface of the antigen presenting cell via its extracellular domain. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Viral protein U (VpU) of HIV-1 plays an important role in downregulation of the main HIV-1 receptor CD4 from the surface of infected cells. Physical binding of VpU to newly synthesized CD4 in the endoplasmic reticulum is an early step in a pathway leading to proteasomal degradation of CD4.
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Product Specification
Basic Information
Brands:Elabscience
Model Number:
Place of Origin:China
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Reconstitution:Please refer to the printed manual for detailed information.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
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Calculated Molecular Weight:42.2 kDa
Observed Molecular Weight:46 kDa
Purity: > 90 % as determined by reducing SDS-PAGE.
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Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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Calculated Molecular Weight:42.2 kDa
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Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
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Application:Cell culture
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Research Areas:immunology;Stem cells
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Bio-activity:Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When cells are added to CD4-coated plates (0.8 μg/mL, 100 μL/well), approximately > 40% will adhere specifically.
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Formulation:Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
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