T4 Endonucleasa V (T4 PDG), 20 KU

Brands:Beyotime

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Place of Origin:Porcelana

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Shipping:Hielo seco

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Component:T4 Endonucleasa V (10 U/µl), tampón de T4 Endonucleasa V 10X

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Purity:≥ 95%

Shelf Life:24 meses

Storage:-20℃

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Test Principle:La endonucleasa V Beyotime T4, también conocida como glicosilasa del dímero de pirimidina T4 (T4 PDG), es una enzima recombinante derivada del fago T4 y actúa como enzima reparadora de daños en el ADN, con actividad de ADN N-glicosilasa y AP-liasa. Su actividad de N-glicosilasa reconoce los dímeros de cis-sin-ciclobutano pirimidina (T^T, T^C, C^C) causados por la radiación UV y escinde el enlace 5'-glicosídico, generando un sitio apurínico/apirimidínico (AP). Su actividad AP-liasa elimina el sitio AP mediante β-eliminación, creando un espacio en el ADN de un nucleótido con extremos aldehído 3'-α,β-insaturados y 5'-fosfato.

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