Kit de ensayo de caspasa-3 GreenNuc™ para células vivas, 100-200 T

Brands:Beyotime

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Shipping:Hielo seco

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Component:Sustrato de caspasa-3 GreenNuc™ (1 mM), Ac-DEVD-CHO (10 mM)

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Shelf Life:1 año a -20 ℃

Storage:-20 ℃

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Em:530 nm

Ex:500 nm

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Test Principle:El sustrato GreenNuc™ Caspasa-3 es un sustrato fluorogénico compuesto por un colorante de ADN y una fracción DEVD, específicamente reconocida por la caspasa 3/7. Este sustrato, con carga negativa e inicialmente no fluorescente, atraviesa la membrana celular y entra en el citoplasma, donde es escindido por la caspasa 3/7 en células apoptóticas para liberar el colorante de ADN fluorescente. El colorante de ADN migra al núcleo celular y se une al ADN para formar el complejo fluorescente verde brillante GreenNuc™-ADN.

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Assay Time:15-3 minutos

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Detection Instrument :Citómetro de flujo, microscopio de fluorescencia o lector de microplacas de fluorescencia

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Form:Líquido

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