Kit de construcción rápida BeyoCRISPR™ (reportero mOrange2), 10 T

Brands:Beyotime

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Place of Origin:Porcelana

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Shipping:Hielo azul/hielo seco

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Component:pU6-gRNA-Cas9-T2A-mOrange2 linealizado (15 ng/μl), agua ultrapura, tampón de construcción rápida 10X, ligasa rápida, oligonucleótido de control ds (1 μM), cebador hU6-F (10 μM)

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Shelf Life:24 meses

Storage:-20℃

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Test Principle:Los usuarios solo necesitan sintetizar oligonucleótidos de ADN específicos para la diana, hibridar para formar dobles cadenas y, a continuación, ligarlos al vector linealizado incluido en este kit para la edición génica de interés basada en CRISPR/Cas9. Tras la transfección, las células de mamífero positivas que expresan Cas9 y ARNg pueden analizarse mediante la fluorescencia naranja-roja mOrange2, donde suele producirse la edición génica.

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